Acids and Bases - Calculating pH of a Strong Base

Acids and Bases - Calculating pH of a Strong Base KOH is an example of a strong base, which means it dissociates into its ions in aqueous solution. Although the pH of KOH or potassium hydroxide is extremely high (usually ranging from 10 to 13 in typical solutions), the exact value depends on the concentration of this strong base in water. So, its important to know how to perform the pH calculation. Strong Base pH Question What is the pH of a 0.05 M solution of Potassium Hydroxide? Solution Potassium Hydroxide or KOH, is a strong base and will dissociate completely in water to K and OH-. For every mole of KOH, there will be 1 mole of OH-, so the concentration of OH- will be the same as the concentration of KOH. Therefore, [OH-] 0.05 M. Since the concentration of OH- is known, the pOH value is more useful. pOH is calculated by the formula pOH - log [OH-] Enter the concentration found before pOH - log (0.05)pOH -(-1.3)pOH 1.3 The value for pH is needed and the relationship between pH and pOH is given by pH pOH 14 pH 14 - pOHpH 14 - 1.3pH 12.7 Answer The pH of a 0.05 M solution of Potassium Hydroxide is 12.7.

Esl And Reading Theories Midterm Essays - Language Education

Esl And Reading Theories Midterm Essays - Language Education Esl And Reading Theories Midterm Mid-Term/Spring 1998 This Termpaper was for an Educational Class for the Teaching Credential Program. The questions listed below describe various theories and questions related to ESL and reading. Hope you find this paper useful. I got an 'A' for this termpaper Bibliography : Author - Ruddell Reading in Secondary Education 1. Krashen's theory of comprehensible input states: We learn a second language containing linguistic structures that are just beyond the structures we already know. (Ruddell, Page 341). According to Krashen, Comprehensible input is symbolized by the following formulated statement: i (input + 1). This means that comprehensible input is just beyond our current level of competence in which we can construct new meanings. Thus, we can construct new meanings with what we already know about language, world knowledge, contextual information, and extralingual information. Krashen also states that we Acquire new structures in a second language not by focusing on the structures themselves, but by understanding the meaning of a communication containing those new structures. (Ruddell, Page 341). Language acquisition environment and language learning environment differ by theory. According to Krashen, Acquisition of a language occurs as a subconscious process as we encounter and use a second language for some communicative purpose. (Ruddell, Page 340). Krashen also states that Conscious learning of a language occurs as we study formally the grammar, structure, and lexicon of a language. (Ruddell, Page 340). Hence, Krashen views acquisition to be the main reason for second-language development. The optimal conditions needed for L2 acquisition, according to Krashen, can be stated as People acquire second languages when they obtain comprehensible input and when their affective filters are low enough to allow the input in. Furthermore, second l language acquisition will occur in classes taught in the second language if the student can understand what is going on in the class; that is, when input is comprehensible. (Ruddell, Page 342). What are two strategies that I can use in my classroom so that language-related problems are not as likely to interfere with the academic aspirations of language minority students? a. I plan on teaching history! Therefore, one strategy I could use is Co-operative learning! Students who have language-related problems can work with other students on projects that I will give in class. These groups can assist those who have language-related problems by working as a team to solve the projects I give them. b. I can also write words from the text that I feel are important to know. For instance, most history books in high school have words that are in bold print. If some of these words are difficult (i.e. - Tyranny, Totalitarian, Anarchy, ect.), I would write these words on the board and introduce those words to the students. I would give the meaning of those words on the board and give examples that correspond to those words. I can even ask them to use these words in sentences if need be! 2. The assumption that language minority students have become English proficient when they have acquired relatively fluent and peer-appropriate face-to-face communicative skills is a misconception. Why? According to Cummins, he estimates that ESL students acquire age-appropriate conversational proficiency (BIC) in about two years, while academic proficiency (CALP) requires 5 to 7 years. (Ruddell, Page 342). Thus, he warns, to Refrain from assuming that second language students' proficiency in conversational English is a true measure of their proficiency in science, or social studies, or mathematics. (Ruddell, Page 342). Cummins also argues that schools placed ESL students in Special Education classes through inappropriate testing practices based on faulty assumptions about a students ability to speak English. Just because an ESL student can speak English fluently doesn't mean that he/she is literate in their understanding of the English language. For example, if I understood Spanish fluently, people would assume that I could get an 'A' in my Spanish classes. Even if I could speak this language easily doesn't mean that I could read or write in that language. Take for another example: illiterate people. That are lots of people in the United States who can speak English easily. But when it comes to reading or writing, they fail. I had one lady ask me if a rubber mat fabric had adhesive on the back side.

Organisational Change Management Essay Example | Topics and Well Written Essays - 3000 words - 11

Organisational Change Management - Essay Example In this regard, the council, through a consultant has been advised to use Appreciative Inquiry 5D to overcome the problem facing the organization and to allow them offer better services to the council. The use of AI 5D involves identifying the strengths rather than weaknesses to bring change in the organization, this paper then discusses the use of AI 5D in bring the necessary change in the council (Englund, Graham, and Paul 2006, p.35). Definition The main concern of the change process in the council is necessitated by the higher levels of absenteeism by the employees who abscond their duties most of the time, they do not show up to take care of their duties and this has led to the emergence of some of the problems associated with absenteeism. The department that was worst hit in this case was that of the elderly care in the council, the care managers then became concerned about the situation as it aggravated the condition of elderly in the council that needed the support of the emp loyees. With the absenteeism rate spiralling, there are a number of issues that became apparent for action to be taken so that change could be brought. One of the salient problems that became out of control was the decline in the quality of the services that was being offered by the council, due to the widespread absenteeism, the quality of the services that was being provided by the council experienced significant drop owing to the fact that there were few employees who showed up. This made it possible for the work to be strenuous to the few individuals who turned up and the demands of the department could not be fully achieved hence decline in the quality of the services (Tennant 2012, p.47). The absenteeism factor also created another problem in the council’s department where the employees who turned up for their duties were subjected to more duties than prescribed so that they could cover for the duties that were to be done by the other workers who decided to be absent. B ecause of these, it became strenuous and stressful to the few workers who maintained reporting for their duties, they worked more than necessary and it was envisaged that if the trend continues, there would be possibility of breakdown and burnout that will definitely impair the performance of the employees who showed up for their duties every day. This consequence too made it prudent for the organization to find a solution to the challenge that was facing the organization as it was bedevilling the performance in more than one way (Reed 2007, p.53). The colleagues that were absent for work had to be covered for by those who turned up, this explains why there was more duties for those who showed up resulting into stressful work (Kluwer 2008, p.58). Lastly, absenteeism also had serious economic consequences for the council, ordinarily employees should be paid for the services that they offer to the organization that employs them, if these services are not offered, then there should be no payments given to the employees. If this happens, there will be obvious losses incurred by the organization, in this respect, the employees that had been out of their duties in the organization were causing serious economic pinch to the organizati

Improvements of the marketing planning process in the pharmaceutical Essay

Improvements of the marketing planning process in the pharmaceutical company - Essay Example Product group should be appealing to the contemporary market. Assuming social responsibility, and employing competent and trained labour forces are also important. Production should be doen in compliance with the EU directives.More aggresive acquisition, promotion and advertising should be undertaken. Company Introduction ACTAVIS AD (formerly known as BALKANPHARMA) a generic pharmaceutical company was founded in 1999 due to privatizations of its three manufacturing sites at Dupnitza, Troyan and Razgrad, with a manufacturing tradition of half a century. It the Bulgarian branch of the MNC generic pharmaceutical group ACTAVIS HF (earlier PHARMACO). The group head quartered in Iceland has its operations in more then 25 countries. Actavis AD is the leading generic pharmaceutical company in Bulgaria enjoying substantial market share both in terms of value and volume. Currently operation and production have been segregated from marketing and sales and Actavis represents the marketing and sales aspect. The ratio of domestic sales and export sales is also significant (51%/49%). It continues to enjoy the traditional favored position both in the domestic market as well as in USSR. The entire capital and is owned by the mother concern and Actavis is the biggest tax payer in Bulgaria. It is the driving force in th e Bulgarian domestic pharmaceutical market. Several restructuring has taken place to divert it focus from the safe and closed market to the more competitive open market. Ongoing efforts to incorporate the Good Manufacturing Practices into its manufacturing facilities are on. To counter foreign and domestic competition various aspects are being developed. ACTAVIS AD in 2002 became the only European pharmaceutical company with a... An analysis of   the market status of Actavis AD   within the Bulgarian pharmaceutical industry, along the significant economic   determinants operating in all markets, namely, supply and demand, barriers to entry and the pricing of goods, production cost and profit, aids in understanding the company’s market status and in devising future strategies.Within the Bulgarian pharmaceutical sector the demand is mostly created by the public funds, namely National Health Reimbursement Fund; hospitals and Ministry of Health centralized tenders. The demand market is differentiated along three parameters, namely those goods which are reimbursed and those which are free sales ones; those which are for sale in hospitals and those which are sold at pharmacies; those which are patented and those which are generic. There is a possibility to enhance the demand in the pharmaceutical market. The supply market can best be represented by the volume in the pharmaceutical market. The market su pply volumes merely indicate the part of the demands which has been satisfied, the actual sales. Actavis Ad enjoys significant market volume (35% in terms of market volume share and 18% in terms of market value share). It emerges as the clear leader outrunning its major business rival Sopharma by 8% in terms of market value share. Despite the small size of the Bulgarian pharmaceutical market(Polish market being ten times bigger) Actavis has recorded a significantly high growth rate attributed to it’s ever expanding portfolio in generic products, commitment.

Virtual Learning Essay Example | Topics and Well Written Essays - 1000 words

Virtual Learning - Essay Example Pursel and Bailey (2005) focus their attention on how online video games can contribute to the elearning processes and stimulate them. They explore a number of resources demonstrating that there are two primary limitations of online learning. Learners generally lack motivation and they do not interact enough during their online courses. Pursel and Bailey (2005) propose that in order to enhance the elearning possibilities new approaches should be adopted by teachers. The new generation processes information in a different way in comparison to their parents', consequently methods used in online games have to be included in the virtual learning. The author's main purpose in writing the article is to offer an alternative solution in overcoming the decreasing interest and value of courses conducted online. Their attempt presents innovative and facilitating model of the learning within the virtual world. Education is a great asset which transforms communication in a higher level. The benefits of good quality online education, however are not available to the urban sections of society. Pursel and Bailey (2005) provide information only about the U.S. consumers and do not refer to sources about the rest of the world. Yajnik (2005) notes that information technology is such a powerful tool that has the potential to make education available even to people in remote locations. The primary limitations lack of interactivity and motivation deficit that Pursel and Bailey (2005) have to be related to a specific strata from the society and clarifications made about the economic status from the country in questions. The generalizations delivered by Pursel and Bailey (2005) show limited research scope. A problematic issue is that the majority of those who drop out of the online courses do not find them challenging enough. It is of critical importance to emphasize that Pursel and Bailey's (2005) pr oposal to incorporate video games strategies into elearning is directed mainly to satisfy the requirements of U.S. student audience. In countries with developing economies students are willing to engage in all kinds of learning to higher their educational background. Yajnik (2005) suggests that the growth in communication technology in India widens the divide between those who have access and those who do not. The main task of elearning methods is not to increase the social gap introducing video games methodology, but to make it possible for underdeveloped countries to have the same opportunities. Implementing video games in the structure of online courses will create immediate exclusion of certain users. E-learning tutors might not want to risk "information exclusion" of their students by complex design or unaffordable software. Kenya's education minister, Professor George Saitoti (cited in Ogodo, 2007) says that when integrated into educational system the Internet Communication technologies "have the capacities to improve the delivery of education through distance learning, provide access to universal digital libraries, offer ways in which universities can globally compete and offer improvements in academic administration (par. 17)." The elearning technologies offer new ways in which the quality and effectiveness of higher education is delivered. However the way in which elearning will be made constructive depends on the

Role Of Metal Ions In Biochemistr

Role Of Metal Ions In Biochemistr A metal is a chemical element that is a good conductor of both electricity and heat and forms cations and ionic bonds with non-metals. In chemistry, ametal (from Greek ÃŽÂ ¼ÃƒÅ½Ã‚ ­Ãƒ Ã¢â‚¬Å¾ÃƒÅ½Ã‚ ±ÃƒÅ½Ã‚ »ÃƒÅ½Ã‚ »ÃƒÅ½Ã‚ ¿ÃƒÅ½Ã‚ ½ mà ©tallon, mine]) is an element, compound, or alloy characterized by high electrical conductivity. In a metal, atoms readily lose electrons to form positive ions (cations). Those ions are surrounded by delocalized electrons, which are responsible for the conductivity. The solid thus produced is held by electrostatic interactions between the ions and the electron cloud, which are called metallic bonds.[2] Metal ions play essential roles in about one third ofenzymes . These ions can modify electron flow I a substrate or enzyme, thus effectively controlling an enzyme-catalyzed reaction. They can serve to bind and orient substrate with respect to functional groups in the active site, and they can provide a site for redox activity if the metal has several valence states. Without the appropriate metal ion, a biochemical reaction catalyzed by a particular metalloenzyme would proceed very slowly, if at all. The enzyme provides an arrangement of sidechain functional groups having an appropriate sized hole with the preferred groups on enzyme side chains needed to bind the required metal ion. The optimal number of such binding groups is chosen for the particular metal ion, together with the appropriate hydrophobic or hydrophilic environment in the binding site. Metal ions may be bound by main-chain amino and carbonyl groups, but specific binding is achieved by the amino acid side chains, particularly the carboxylate groups of aspartic and glutamic acid, and the ring nitrogen atom of histidine. Other side chains that bind metals ions include tryptophan (ring nitrogen), cysteine (thiol), methionine (thioether), serine, threonine, tyrosine (hydroxyl groups), and asparagine and glutamine (carbonyl groups, less often amino group . No set of general rules exists that describes how a given metal ion will behave in an enzyme . Now that many crystal structures of proteins are being studied by X-ray diffraction, information on the binding of metal ions in the active sites of enzymes is available and should provide clues to the mechanism of action of the enzyme.The examples of catechol methyltransferase andmandelate racemase will be discussed later in this article.The work described here includes results fromexaminations of the crystal structures in the CambridgeStructural Database and the Protein Databank . Astudy of binding, however, also involves an analysis ofthe energetic consequences of changing the way thebinding occurs, so that the most stable binding pattern fora given group of ligands can be deduced. We haveapproached this using ab initio molecular orbital and density functional calculations . In this way weobtain both the binding geometry of ligands and theenergetic consequences of changing this binding m ode. Properties of metal ions Metal ions are generally positively charged and act as electrophiles, seeking the possibility of sharing electron pairs with other atoms so that a bond or charge-charge interaction can be formed. They behave rather like hydrogen ions (the poor mans metal). Metal ions, however, often have positive charges greater than one,and have a larger ionic volume so that they can accommodate many ligands around them at the same time. In addition, metal ion concentrations can be high atneutral pH values, while hydrogen ion concentrations are, by the definition of pH, low at these values. Ligands are the atoms or groups of atoms that are bonded to the metal ion, generally in an electrostatic manner. They are usually neutral or negatively charged and they donate electron density to the metal ion. Thecoordination number of a metal ion, that is, the number of ligand atoms bound to it, is viewed in terms of concentric spheres; the inner sphere containing those atoms in contact with the metal ion, the second sphere containing those in contact with the inner sphere ligand atoms. The number of atoms in these spheres will depend on the size of the metal ion and the sizes of the ligand atoms. For example, sodium is smaller than potassium, and sulfur is larger than oxygen. Measurements of metal ion-liganddistances in crystal structures led to the idea of atomic and ionic radii [9-11]; anion radii can also be derived from the minimum anion-anion distances in crystal structures. The radius ratio, a concept introduced by Goldschmidt [11], is the ratio of the radius of the cation to that of the anion and is generally less than 1.0 Tetrahedral structures have a radius ratio between 0.225 and 0.414, while octahedral structures have a ratio between 0.414 and 0.645. For example, the radius of Mg2+ is 0.65 D, while that of O2- is 1.40 D and their radius ratio is 0.464; the packing is octahedral. The charge distribution in the active site of an enzyme is designed to stabilize the transition state of the catalyzed reaction relative to that of the substrate. In enzyme-catalyzed reactions it is essential that the reactants be brought together with the correct spatial orientation, otherwise the chance of the reaction taking place is diminished and the reaction rate will be too low.The electrostatic environment in the active site is a major factor that serves to guide the substrate to the binding site in the correct orientation. Metal ions can assist in this process, often binding groups in a stereochemically rigid manner, thereby helping to control the action of the enzyme. Thus, an enzyme will bind its substrate in such a manner that immobilization and alignment, ready formation of the transition state of the reaction to be catalyzed,and then easy release of the product will result; metal ions often help in accomplishing this process. Each metal ion has its own chemistry. An example of the differing reactivities of metal cations is provided by their ability to bind or lose water molecules. The exchange of coordinated water with bulk solvent by various cations has been categorized into four groups: those for which the exchange rate is greater than 108 per second including alkali and alkaline earth metal ions(except beryllium and magnesium), together with Cr3+,Cu2+, Cd2+, and Hg2+. Intermediate rate constants (from 104 to 108 per second) are found for Mg2+ and some of the divalent first-row transition metal ions. Those with slow rate constants (from 1 to 104 per second) include Be2+ and certain trivalent first-row transition metal ions. The inert group with rates from 10-6 to 10-2 per second containsCr3+, Co3+, Rh3+, Ir3+, and Pt2+. One of the factors involved in rates of exchange is the charge-to-radius Ratio; if this ratio is high the exchange rate is low.An important reaction catalyzed by metal ions inenzymes is the ionization of water to give a hydrated hydrogen ion and a hydroxyl anion. Initial studies of this process will be discussed here as they are relevant to the action of a metal ion in providing a hydroxyl group and a hydrogen ion for use in an enzymatic reaction. Polarizing Potential of Various Ions Atoms or groups of atoms are considered polarizable if, when they are placed in an electric field, a charge separation occurs and a dipole is acquired. This deformability or polarizability is measured by the ratio of the induced dipole to the applied field. Those atoms that hold on less firmly to their electrons are termed more polarizable. It is found that if two ions have the same inert gas structure (potassium and chloride, for example), the negatively charged anion is more polarizable than the positively charged cation, which holds on to its electrons more tightly. The word hard has been introduced to indicate a low polarizability so that the electron cloud is difficult to deform (like a hard sphere). By contrast soft means high polarizability so that the electron cloud is readily deformed . A hard acid or metal cation holds tightly to its electrons and therefore its electron cloud is not readily distorted; its unshared valence electrons are not easily excited. Soft (polarizable) metal cations contain electrons that are not so tightly held and therefore are easily distorted or removed. A hard acid prefers tocombine with a hard base, while a soft acid prefers to bind with a soft base by partially forming covalent bonds .The type of binding is related to the highest occupied molecular orbital (HOMO) of the electron-pair donor (a lewis base, the ligand) and the lowest unoccupied molecular orbital (LUMO) of the electron-pair acceptor (a Lewis acid, the metal ion). If these have similar energies, then electron transfer will give a covalent (soft) interaction, whereas the energy difference is large, electron transfer does not readily take place and the interaction is mainly electrostatic (hard-hard). Hardcations include the alkali and alkaline earth metal ions while soft metal ions include Cu 2+, Hg2 2+, Hg2+, Pd2+. Inbiological systems, hard ligands generally contain oxygen while soft ligands contain sulfur. Hard acids tend to bind hard bases by ionic forces, while soft acids bind soft bases by partially forming covalent bonds. These hard-soft categorizations are a help in understanding the relative binding preferences of various cations. Most metal ions of biological significance are hard or intermediate between hard and soft. Most soft metal ions and soft ligands are poisonous and they interact with other soft species in the body. For Pb2+ the harder ligands are found in hemidirected structures and the softer ligands in holodirected complexes.Nature has devised many enzyme systems in which a metal ion interacts with the oxygen of a water molecule.If a water molecule can be dissociated into a hydrogen ion and a hydroxyl group, the latter can serve as a nucleophile in chemical a nd biochemical reactions.Nature has chosen activation of a water molecule as a means to obtain such a nucleophile in situation so that a chemical reaction can occur in a stereochemically controlled manner in the active site of the enzyme. The questions we ask are as follows: 1) how does nature ensure that the specific water molecule will be activated; 2) how does nature compensate for the lower water activation power of some cations over others (since a wide variety of metal ions may not be available in the particular active site and the enzyme has to do the best it can with what is available); and 3) how does nature ensure that the required reaction occurs. Ab initio molecular orbital and density functional calculations have been carried out to measure the extent to which a series of metal cations can, on binding with water, cause it to be dissociated into its component hydrogen ions (subsequently hydrated in solution) and hydroxyl ions. Initial data indicate that the charge of the metal ion plays a significant role in modifying the pKa of water. The binding enthalpies of a wide variety of metal ion monohydrates, M[H2O]2+ , have been published [21] but their deprotonation enthalpies are still under investigation. Geometry of Metal-Ion Binding to Functional Groups The geometries of metal ion-carboxylate interactions have been studied in order to determine the following: 1)which lone pair of an oxygen atom in a carboxylate group, syn or anti, is preferred for metal cation binding; 2) does the metal ion lie in the plane of the carboxylgroup; and 3) under what conditions do metal ions share both oxygen atoms of the carboxylate group equally? We found that cations generally lie in the plane of the carboxylate group . The exceptions to this mainly include the alkali metal cations and some alkaline earth cations; these metals ionize readily and form strong bases so it is not surprising that they have less specific binding modes. When the distance of the metal cation to the carboxylate oxygen atoms is on the order of 2.3-2.6 D, the metal ion tends to share both oxygen atoms equally. Otherwise one oxygen atom of the carboxylate group is bound to the metal ion and the other is not. Calcium ions often form bidentate interactions, while it is less common for the smaller magnesium ions. Imidazole groups in histidyl side chains of proteins bind metal ions in a variety of enzymes. One imidazole can, by virtue of its two nitrogen atoms, bind one or two metal ions, depending on its ionization state and the suitabilities of the metal ion. The bases in DNA can also bind metal ions. We have analyzed hydrogen bonding to and from nitrogen atoms in nitrogen-containing heterocycles for crystal structures in the Cambridge Structural Database. It was found that for hydrogen bonding, a slight out-of-plane deviation of the binding atom often occurs. Metal ions bind more rigidly in the plane of the imidazole group. The energetic cost of such deviations were analyzed by ab initio molecular orbital calculations. In an investigation of protein crystal structures in the Protein Databank it was found that the binding of metal ions to histidine in proteins is more rigid and the location of the metal ion is more directional. Thus, if an enzyme needs to control the location and orientation of a carboxylate or imidazole group, it can accomplish this better with a metal ion than by hydrogen bonding. Metal ions in proteins are often involved in structural motifs. When a metalloenzyme carries out its catalytic function it uses one of a few possible three-dimensional arrangements of functional groups around the metal ion to ensure the specificity of the required biochemical reaction. Thus, if such catalytic metal-binding motifs can be identified and categorized, then incipient reactivities of enzymes could be inferred from their three-dimensional structures. Such a categorization, however, requires an understanding of the underlying chemistry of any metal ion in the active site. One motif identified in the crystal structure of cobalt(II) formate consists of a carboxyl group in which one oxygen atom is bound to the metal ion and the other is bound to metal-bound water, to give a cyclic structure. This motif has been found in many metalloenzyme crystal structure , such as D-xylose isomerase . The roles of these motifs are of interest. The metal ion-hydrated-carboxylate motif (I) is planar and commonly found. It does not, however, affect the ability of the metal ion (in studies of Mg2+ complexes) to ionize water. On the other hand, for magnesium ions (which generally have a rigid octahedral arrangement of binding groups) it utilizes 2 of the 6 coordination positions and therefore serves to orient the arrangement of ligands, an effect we have labeled coordination clamping. Motif (II) is also found in several crystal structures such as that of the -subunit of integrin CR3 . It appears to help bind subunits together. A third motif (III) is found in D-xylose isomerase and involves two metal ions with several carboxylate ligands and a histidine ligand . The metal site that binds only oxygen atoms can bind substrate in place of the two water molecules and orient the substrate. The second metal ion site (with histidine as one ligand) then positions a metal ion-bound water molecule to attack the substrate. Roles of Metal Ions in Enzyme Action The crystal structure of mandelate racemase with bound p-iodomandelate provides a useful example of the importance of a metal ion in a reaction . The enzyme binds a magnesium ion by means of three carboxyl groups. The substrate mandelate has displaced water from the magnesium coordination sphere and binds by means of its carboxylate group and an a-hydroxy group.The magnesium ion will lie in the plane of the carboxyl group, as shown by our studies of metal ion-carboxylate interactions . The magnesium holds the substrate firmly in place so that the catalytic abstraction and addition of a hydrogen atom by His 297 or Lys 166 is precisely effected . The magnesium probably also aids this activity by affecting the electronic flow in the carboxylate and hydroxyl groups by mild polarization. We have found that metal ion coordination is better than a hydrogen bond in aligning a functional group; there is considerable flexibility in a hydrogen bond as we found for imidazoles . In the reaction c atalyzed by the enzyme mandelate racemase the magnesium ion binds substrate . A Histidine (His 297) and Lysine (Lys 168) are positioned to abstract a hydrogen ion from the substrate and, if it is added again from the other side, racemization occurs. Hydrogen bonding to a carboxylate group of the substrate helps to stabilize an enolate intermediate in the reaction. In catechol O-methyltransferase , a methyl group is transferred from the sulfur of Sadenosy[ methionine to catechol. The magnesium ion is oriented by a motif of type I and it binds substrate in such an orientation that a hydroxyl group is near the S-CH3 group, and the other hydroxyl group is held in place by a carboxylate group. There are many other examples of two-metal ion active sites, such as hemerythrin, alkaline phosphatase and superoxide dismutases (which have been well documented). These studies of the geometries and energetics of metal-ion ligand b inding can therefore aid in our understanding of metalloenzyme function Metals in the RNA worid By combining our limited knowledge of metal-ion-binding to contemporary RNAs and our more extensive knowledge of metal-ion-binding to proteins, it is possible to speculate on the role of metal ions in prebiotic molecular evolution. It seems clear that specifically bound metal ions coevolved with RNA molecules. Many of the mononuclear sites in Table 5 are formed with, or can be engineered into, small RNA fragments. Since such sites are highly hydrated and contain limited direct contact with the RNA, the observed affinities are only moderate, in the 1-1000 ÃŽÂ ¼M range. These sites are also expected to show limited specificity, predominantly dictated by the chemical nature of the ligands. Furthermore, in these examples, the RNA structures themselves are likely to be quite flexible and can accommodate a variety of metal ions with only minor distortions to the overall RNA fold. These minimalist sites are sufficient to stabilize the secondary and tertiary structures observed in these motifs. The metal ion sites generated on small RNAs appear to be capable of facilitating a variety of different types of chemistry. Activities range from the transesterification and hydrolytic reactions of small ribozymes (Pyle 1996; Sigurdsson et al. 1998) to the more exotic porphyrin metalation (Conn et al. 1996) and Diels-Alder condensation reactions (Tarasow et al. 1997) catalyzed by aptamers produced from in vitro selection experiments.These small RNAs have only limited amounts of structure and therefore are likely to position the catalytic metal ions by only a few points of contact. The relatively modest rate enhancements supported by catalytic RNAs such as these probably reflect the types of species that first evolved from random polymerization events. Very active metal ions might have assisted in this process but would have increased the danger of side reactions that would accidentally damage the catalyst. A striking difference between most RNA metal-binding sites studied thus far and those seen in proteins is the degree of hydration. Both structural and catalytic metal-ion-binding sites in proteins are predominantly dehydrated (Lippard and Berg 1995). Water molecules occasionally appear in the coordination spheres of these metal ions, but in these cases, they are often believed either to be displaced by the substrate when it enters the active site or to take part in the catalytic mechanism of the enzyme. Such protein sites also bind their metal ions much more tightly than the RNA systems. In fact, tight binding is a requirement for dehydrated sites, since there is a characteristic energy (ÄHhyd) associated with the hydration of any ion. The net binding energy upon coordination of the ion must account for the energetic cost of dehydration. The question arises, Why are such dehydrated sites not observed in RNAs? One possibility is that metal-binding sites in RNAs are intrinsically different from those in proteins. RNA has a much more limited set of ligands to use in generating a specific metal-binding pocket. Amino acid side chains containing thiols and thioethers are well suited to binding a variety of softer metals. In addition, the carboxylate side chains provide anionic ligands with great versatility in their potential modes of coordination. They can act as either terminal or bridging ligands and bind in either monodentate or bidentate geometries. The nucleotides, on the other hand, are much larger and more rigid than the corresponding amino acids. The anionic ligand in RNA, the nonbridging phosphate oxygen, is an integral component of the backbone and therefore is more limited in its conformational freedom than the aspartate and glutamate carboxylate groups. The heterocyclic ring nitrogens and the keto oxygens from the bases are held in rigidly planar orientations by the aromatic rings. This geometric constraint severely limits the ability of an RNA to compact encompass a metal ion and provide more than facial coordination and therefore complete dehydration. It also explains why the most specific metal-binding sites are not in the Watson-Crick base-paired regions of the structure where the conformation is too constrained. Instead, metalion- binding sites are clustered in regions of extensive distortion from the A-form RNA helices. There is also the question of the folding of RNAs relative to that of proteins. It is possible that in RNAs there is insufficient energy in the folding and metal-binding process to completely displace the waters of hydration around a metal ion. It has been suggested that in contemporary RNAs, modified nucleotides might be present to assist in metal ion binding (Agris 1996). A more straightforward possibility, however, is that most RNAs studied to date are structurally too simple. In these RNAs, most residues involved in metal ion binding are solvent-exposed. Thus, the RNAs have no real inside comparable to the hydrophobic core of a protein. The largest RNA crystallographically characterized to date is the P4-P6 domain. On the basis of that structure, it was proposed that an ionic core may substitute in RNA folding for the hydrophobic core of proteins such that the 3 ° structure assembles around a fixed number of discrete metal-binding sites (Cate et al. 1997). Even in this structur e, however, the most buried of the metal-binding sites are significantly hydrated. It could be that all metal-ion-binding sites in RNA are at least partially hydrated. One can imagine several advantages to using hydrated ions within the ionic core of a large RNA. Hydrated ions would span larger voids than dehydrated ions and allow looser packing of secondary structure elements. The hydrated ion also can accommodate a wide range of structural interactions through its orientation of the water molecules as compared to direct coordination of metal ions at every site. In addition, the energy associated with deforming the outer-sphere interactions should be significantly less than what would be observed for distorting the innersphere coordination. A consequence of RNAs having a core of hydrated ions is that one might expect this core to be much more dynamic than the hydrophobic core of a protein. In the modern protein world, metal cofactors are associated with a variety of reaction types, including electron transfer, redox chemistry, and hydrolysis reactions. Trans esterification and hydrolytic activities, however, are the primary catalytic behaviors observed in ribozymes. Did these other catalytic activities not develop until the dawn of the protein world, or are there undiscovered natural catalytic RNAs that are the ancestors of the early redox enzymes? Through the use of in vitro selection experiments, the scope of RNA catalysis has been significantly broadened is almost certainly capable of catalyzing these other classes of reactions, but it is still unclear whether there are naturally occurring examples. Such an enzyme would likely use a metal ion cofactor other than Mg(II), so the search for RNA molecules that naturally use alternative ions is of significant interest. A recent selection experiment showed that a single base change results in an altered metal ion specific ity for RNase P (Frank and Pace 1997). It is clear from this result that catalytic RNAs retain the ability to adapt to an everchanging environment, using the resources available to evolve and to overcome evolutionary pressures. Were RNAs to have evolved out of an environment devoid of metal ions, they probably would have found a way around the problems of folding and generating reactive functional groups. The primordial soup and all cellular environments that have evolved subsequently contained a variety of ions, however. Given the availability of metal ions, they will certainly play a significant role in the biology of current and future RNAs. Effect of metal ions on the kinetics of tyrosine oxidation by Tyrosinase The conversion of tyrosine into dopa [3-(3,4-dihydroxyphenyl)alanine] is the rate limiting step in the biosynthesis of melanins catalysed by tyrosinase. This hydroxylation reaction is characterized by a lag period, the extent of which depends on various parameters, notably the presence of a suitable hydrogen donor such as dopa or tetrahydropterin. We have now found that catalytic amounts of Fe2+ ions have the same effect as dopa in stimulating the tyrosine hydroxylase activity of the enzyme. Kinetic experiments showed that the shortening of the induction time depends on the concentration of the added metal and the nature of the buffer system used and is not suppressed by superoxide dismutase, catalase, formate or mannitol. Notably, Fe3+ ions showed only a small delaying effect on tyrosinase activity. Among the other metals which were tested, Zn2+, Co2+, Cd2+ and Ni2+ had no detectable influence, whereas Cu2+ and Mn2+ exhibited a marked inhibitory effect on the kinetics of tyrosine ox idation. These findings are discussed in the light of the commonly accepted mechanism of action of tyrosinase. Tyrosinase (monophenol,dihydroxyphenylalanine oxygen oxidoreductase; is a copper-containing enzyme responsible for melanogenesis in plants and animals, which catalyses both hydroxylation of tyrosine to dopa and its subsequent oxidation to dopaquinone (Hearing et al., 1980; Lerch, 1981). The first reaction, which represents the rate-limiting step in melanin biosynthesis (Lerner et al., 1949), is characterized by a lag period that has subsequently been explained in terms of a hysteretic process of the enzyme (Garcia Carmona et al., 1980). The extent of this induction time depends on various parameters including, besides pH and both substrate and enzyme concentration, the presence of a suitable hydrogen donor. Kinetic studies carried out on tyrosinases from various sources (Pomerantz, 1966; Pomerantz Murthy, 1974; Hearing Ekel, 1976; Prota et al Abbreviations used: dopa, 3-(3,4-dihydroxyphenyl)-alanine; SOD, superoxide dismutase. To whom correspondence and reprint requests should be addressed. 1981) have shown that dopa, in very low concentration, is the most effective reducing agent in eliminating the lag period, whereas other catechols, such as dopamine, adrenaline and noradrenaline, behave similarly to ascorbate and NADH and NADPH in only shortening it, even at high concentration. Tetrahydropterin, a well-known specific cofactor of other aromatic hydroxylases (Lerner et al., 1977; Marota Shiman, 1984), is also effective in stimulating tyrosinase activity, although to a lesser extent than dopa. At present, no other organic or inorganic substances have been reported to shorten or lengthen the lag period of tyrosine oxidation. Although metal ions are known to play a role in many biologi cal processes, little attention has been directed to their possible involvement in melanogenesis, particularly in the early enzymic stages .As a part of our continuing studies on the chemistry of melanin pigmentation (Prota, 1980; Sealey et al., 1982; Palumbo et al., 1983), we report the results of a survey on the effect of metal ions on the activity of purified Sepia tyrosinase, readily available in large amounts from the ink of the cephalopod Sepia officinalis thermostability of amalyse Three Metal Ions Participate in the Reaction Catalyzed by T5 Flap Endonuclease*à ¢- ¡ Protein nucleases and RNA enzymes depend on divalent metal ions to catalyze the rapid hydrolysis of phosphate diester linkages of nucleic acids during DNA replication, DNA repair, RNA processing, and RNA degradation. These enzymes are widely proposed to catalyze phosphate diester hydrolysis using a two-metal-ion mechanism. Yet, analyses of flap endonuclease (FEN) family members, which occur in all domains of life and act in DNA replication and repair, exemplify controversies regarding the classical two-metal-ion mechanism for phosphate diester hydrolysis. Whereas substrate-free structures of FENs identify two active site metal ions, their typical separation of>4 AËÅ ¡ appears incompatible with this mechanism. To clarify the roles played by FEN metal ions, we report here a detailed evaluation of the magnesium ion response of T5FEN. Kinetic investigations reveal that overall the T5FEN-catalyzed reaction requires at least three magnesium ions, implying that an additional metal ion is bound. The presence of at least two ions bound with differing affinity is required to catalyze phosphate diester hydrolysis. Analysis of the inhibition of reactions by calcium ions is consistent with a requirement for two viable cofactors (Mg2_ or Mn2_). The apparent substrate association constant is maximized by binding two magnesium ions. This may reflect a metal dependent unpairing of duplex substrate required to position the scissile phosphate in contact with metal ion(s). The combined results suggest that T5FEN primarily uses a two-metal-ion mechanism for chemical catalysis, but that its overall metallobiochemistry is more complex and requires three ions. Key cellular processes such as DNA replication, DNA repair, RNA processing, and RNA degradation require the rapid hydrolysis of the phosphate diester linkages of nucleic acids. The uncatalyzed hydrolysis of phosphate diesters under biological conditions is an extremely slow process with an estimated half-life of 30 million years at 25  °C (1). Protein nucleases and RNA enzymes produce rate enhancements of 1015-1017 to allow this reaction to proceed on a biologically useful time scale. Most enzymes catalyzing phosphate diester bond hydrolysis have a requirement for divalent metal ions. Based largely upon crystallographic observations, most metallonucleases are proposed to catalyze reactions using a two-metal-ion mechanism (Fig. 1a) analogous to that suggested for the phosphate monoesterase alkaline phosphatase (2, 3), although this view is not universally accepted. Three recent reviews present contrasting views on the roles of metal ions in protein nuclease and RNA enzyme reactions and illustrate this controversy (4-6). One family of metallonucleases over which there has been considerable mechanistic debate are the flap endonucleases (FENs)3 (7-12), which are present in all domains of life and play a key role in DNA replication and repair. Unlike most metallonucleases, which typically possess a cluster of three or four active site carboxylates, the FEN active site is constructed from seven or eight acidic residues located in similar positions in FENs from a range of organisms (Fig. 1b, see also supplemental Fig. S1) (7, 9, 10, 13-16). Several FEN x-ray structures also contain two active site carboxylate-liganded divalent metal ions, designated as metals 1 and 2 (9, 13-15). The position of metal 1 is similar in all cases, but the metal 2 location varies. In all but on

Henrik Isbens A Dolls House Essay -- A Dolls House Essays

  Ã‚  Ã‚  Ã‚  Ã‚  A Doll’s House, a play by Henrik Ibsen, tells the story of Nora, the wife of Torvald Helmer, who is an adult living as a child, kept as a doll by her husband. She is expected to be content and happy living in the world Torvald has created for her. By studying the play and comparing and contrasting the versions presented in the video and the live performance, one can analyze the different aspects of it.   Ã‚  Ã‚  Ã‚  Ã‚  Ibsen’s purpose for writing this piece is to entertain while pointing out an injustice. Through the events of the play, Nora becomes increasingly aware of the confines in which Torvald has placed her. He has made her a doll in her own house, one that is expected to keep happy and busy as a songbird, who acts and does as he deems proper. As a result of this, she is often pointed out to be very simple by the other characters. Her friend Christina calls her â€Å"a mere child,† showing how naà ¯ve she appears to be to the hardships in life. To prove to her friend that she really has achieved something on her own to be proud of, Nora tells Christina of her secret borrowing of money for the trip to Italy that saved Torvald’s life. Everyone believed that Nora had gotten the money from her father, while actually she found someone to borrow the money from and had been paying her debt back. She did so by spending frugally and always saving some of the money Torvald had given her and by doing odd jobs. She explained to Christine, When Torvald gave me money for clothes and so on, I never spent more than half of it; I always bought the simplest things†¦and besides that, I made money in other ways. Last winter†¦I got a heap of copying to do. I shut myself up every evening and wrote far into the night†¦[I]t was splendid to work in that way and earn money. I almost felt as if I was a man. Later, while discussing his illness with her, Dr. Rank actually comments that Nora is â€Å"deeper than†¦[he] thought.† He too looked at her as like a child. The climax of the story comes when Torvald learns of Nora’s forgery and yells angrily at her. He then finds the promissory note, returned by Krogstad, and realizes that no one has anything over his head any longer. During this episode, Nora realizes what has been going on: that she has become Torvald’s â€Å"doll† which plays around his â€Å"doll† house. She points out to him: You have never under... ...le â€Å"lark.† She spends her days shopping and playing with the children. Also, the characters, locales, and scenes are limited. The characters in this play number only eleven: Nora, Torvald, the three Helmer children, Dr. Rank, Christina, Anna, Krogstad, Ellen, and a porter. The live stage production did not even include the children, as they were not essential to the action of the play. There was but one set in the stage production, and few more in the video, and the play has only three acts. Finally, the construction is tight. There are few, if any, loose ends at the conclusion of the play. Nora reveals her true feelings to Torvald in an exciting scene, Christina deals with unresolved situations with Krogstad, and Dr. Rank tells the Helmers good-bye. These all neatly tie together the previous conflicts.   Ã‚  Ã‚  Ã‚  Ã‚  It is interesting to see how these elements---purpose, point of view, genre, style, motivation, and structure---make up the underlying pieces of the play. Without them, the play becomes little more than a pointless story with which the audience cannot identify. Even with these common pieces, different versions show us different twists on the same play. Henrik Isben's A Doll's House Essay -- A Doll's House Essays   Ã‚  Ã‚  Ã‚  Ã‚  A Doll’s House, a play by Henrik Ibsen, tells the story of Nora, the wife of Torvald Helmer, who is an adult living as a child, kept as a doll by her husband. She is expected to be content and happy living in the world Torvald has created for her. By studying the play and comparing and contrasting the versions presented in the video and the live performance, one can analyze the different aspects of it.   Ã‚  Ã‚  Ã‚  Ã‚  Ibsen’s purpose for writing this piece is to entertain while pointing out an injustice. Through the events of the play, Nora becomes increasingly aware of the confines in which Torvald has placed her. He has made her a doll in her own house, one that is expected to keep happy and busy as a songbird, who acts and does as he deems proper. As a result of this, she is often pointed out to be very simple by the other characters. Her friend Christina calls her â€Å"a mere child,† showing how naà ¯ve she appears to be to the hardships in life. To prove to her friend that she really has achieved something on her own to be proud of, Nora tells Christina of her secret borrowing of money for the trip to Italy that saved Torvald’s life. Everyone believed that Nora had gotten the money from her father, while actually she found someone to borrow the money from and had been paying her debt back. She did so by spending frugally and always saving some of the money Torvald had given her and by doing odd jobs. She explained to Christine, When Torvald gave me money for clothes and so on, I never spent more than half of it; I always bought the simplest things†¦and besides that, I made money in other ways. Last winter†¦I got a heap of copying to do. I shut myself up every evening and wrote far into the night†¦[I]t was splendid to work in that way and earn money. I almost felt as if I was a man. Later, while discussing his illness with her, Dr. Rank actually comments that Nora is â€Å"deeper than†¦[he] thought.† He too looked at her as like a child. The climax of the story comes when Torvald learns of Nora’s forgery and yells angrily at her. He then finds the promissory note, returned by Krogstad, and realizes that no one has anything over his head any longer. During this episode, Nora realizes what has been going on: that she has become Torvald’s â€Å"doll† which plays around his â€Å"doll† house. She points out to him: You have never under... ...le â€Å"lark.† She spends her days shopping and playing with the children. Also, the characters, locales, and scenes are limited. The characters in this play number only eleven: Nora, Torvald, the three Helmer children, Dr. Rank, Christina, Anna, Krogstad, Ellen, and a porter. The live stage production did not even include the children, as they were not essential to the action of the play. There was but one set in the stage production, and few more in the video, and the play has only three acts. Finally, the construction is tight. There are few, if any, loose ends at the conclusion of the play. Nora reveals her true feelings to Torvald in an exciting scene, Christina deals with unresolved situations with Krogstad, and Dr. Rank tells the Helmers good-bye. These all neatly tie together the previous conflicts.   Ã‚  Ã‚  Ã‚  Ã‚  It is interesting to see how these elements---purpose, point of view, genre, style, motivation, and structure---make up the underlying pieces of the play. Without them, the play becomes little more than a pointless story with which the audience cannot identify. Even with these common pieces, different versions show us different twists on the same play.

Clinic Management System

Description this is a medical management system, assisting user to administer a huge data in clinic. In addition, another function is allowing doctor, nurses and the administrative staff. On the client point of view, this is a faster and easeful way to link to the healthcare service by using the system. ‘Clinic Management System – CMS’ is specially designed for general clinic, this system let them have high efficiency management tools, computerize and systematic patient’s record, detail of drug information, this is the first achievements of the medical services. Medical services computerize is an irresistible general trend, this web site will provide medical information for the client, the user can find out a message they care. CMS provide on line appointment feature, which allow patients to make the appointment through Internet. Furthermore, doctors can manage the clinic daily work by using CMS. Nowadays computer has a strong capacity to provide information on a person that has a disease or has a health problem that needs to be cure, a lot of people are going to their doctor and asking them on their status, and a lot of doctors are always saying to us do you have record in this hospital, if the person then the doctor well go and find that record on the cabinet and by looking for you files it will take a lot minutes, that’s way this program came out a system that will easily search the medical records of the patients, in this case this program has database that record the status of the patients, it is easily use to find out the status of a person simple clicking their files or searching their status on the database of the hospital, first we need to identify the person by knowing who is he/she by filling up the form that was provided by the hospital. VI. DEFINITATION OF TERMS Once data have been screened for typographical errors, the data can be validated to check for logical errors. An example is a check of the subject's date of birth to ensure that they are within the inclusion criteria for the study. These errors are raised for review to determine if there are errors in the data or if clarifications from the investigator are required. Another function that the CDM can perform is the coding of data. Currently, the coding is generally centered around two areas adverse event terms and medication names. With the variance on the number of references that can be made for adverse event terms ormedication names, standard dictionaries of these terms can be loaded into the CDM. The data items containing the adverse eventterms or medication names can be linked to one of these dictionaries. The system can check the data in the CDM and compare them to the dictionaries. Items that do not match can be flagged for further checking. Some systems allow for the storage of synonyms to allow the system to match common abbreviations and map them to the correct term.

Free Essays on Norman Rockwell

Norman Rockwell Norman Rockwell is a well known illustrator and artist. He proved to be very diverse and apt to drawing what he saw in the world. He would quote â€Å"I paint life as I would like it to be. If there were problems in this lifetime they were humorous problems.† This would go to show how Rockwell was funny and creative with every drawing. Rockwell was well known for his drawings in the Saturday Evening Post, which was a major magazine in the 1920’s. He was very successful when he was young and proved to be very successful as he got older. He lived a full and complete life full of drawings and major illustrations. Throughout this paper we will describe some of his artwork, tell you about his life, family, and major accomplishments he was able to receive and achieve in his lifetime. Norman Rockwell was bon on February 3, 1894 in New York City. His family resided in New York until Norman was nine then the family moved to Mamaroneck. Norman began taking weekly leave from high school while he was a freshman to attend the Chase School of Art on a part-time basis. He would leave high school for good as a sophomore to study art at the National Academy of Design. It wasn’t until 1911 that Rockwell would get his first assignment to illustrate a children’s book. In 1913 he was named art editor for Boy’s Life at just 19 years old. On May 20, 1916, he illustrated his first Saturday Evening Post cover which would be his big break into the business. By 1920 Rockwell was the Post’s top cover illustrator and by 1925 he had become a national name. By the time Rockwell was twenty-one he illustrated for such magazines as Life, Literary Digest, and Country Gentleman. Rockwell first marriage ended in 1930 and a year later he was remarried to Ma ry Barstow. They had three sons together, Jarvis, Thomas, and Peter. After Mary’s death in 1959, Rockwell remarried for a third time in 1961. He would remain married ... Free Essays on Norman Rockwell Free Essays on Norman Rockwell Norman Rockwell Norman Rockwell is a well known illustrator and artist. He proved to be very diverse and apt to drawing what he saw in the world. He would quote â€Å"I paint life as I would like it to be. If there were problems in this lifetime they were humorous problems.† This would go to show how Rockwell was funny and creative with every drawing. Rockwell was well known for his drawings in the Saturday Evening Post, which was a major magazine in the 1920’s. He was very successful when he was young and proved to be very successful as he got older. He lived a full and complete life full of drawings and major illustrations. Throughout this paper we will describe some of his artwork, tell you about his life, family, and major accomplishments he was able to receive and achieve in his lifetime. Norman Rockwell was bon on February 3, 1894 in New York City. His family resided in New York until Norman was nine then the family moved to Mamaroneck. Norman began taking weekly leave from high school while he was a freshman to attend the Chase School of Art on a part-time basis. He would leave high school for good as a sophomore to study art at the National Academy of Design. It wasn’t until 1911 that Rockwell would get his first assignment to illustrate a children’s book. In 1913 he was named art editor for Boy’s Life at just 19 years old. On May 20, 1916, he illustrated his first Saturday Evening Post cover which would be his big break into the business. By 1920 Rockwell was the Post’s top cover illustrator and by 1925 he had become a national name. By the time Rockwell was twenty-one he illustrated for such magazines as Life, Literary Digest, and Country Gentleman. Rockwell first marriage ended in 1930 and a year later he was remarried to Ma ry Barstow. They had three sons together, Jarvis, Thomas, and Peter. After Mary’s death in 1959, Rockwell remarried for a third time in 1961. He would remain married ...

Free Essays on Flood Comparison

The Chaldean Flood Tablets, spawned from the city of Ur (Southern Iraq), depicts how the Babylonian God, Ea (a god who took part in the creation of man), rendered â€Å"the end of all flesh.† Nearly identical to the way God covered the earth with a great rain to spite the lawlessness of man. The biblical and the Babylonian interpretations resemble each other in many aspects though differ in distinct parts. Mankind became obnoxious, mendacious, and sinful in the eyes of God. Comparable to the Babylonian story, the people were looked upon as being too numerous and too noisy. God chose a righteous man named Noah to carry on the human race after The Great Flood. The Babylonian god Ea chose Utnapishtim to do the same, indirectly warning Utnapishtim of the great revelation through a dream. In contrast, Noah was forewarned directly through the instructions of God. Noah was told to build an ark of gopher wood and cover it inside and out with a pitch, just as Utnapishtim was told. The arks had an opening for daylight, a single door, along with numerous internal compartments. Noah’s ark was constructed 3 stories high and rectangular shaped opposed to the Babylonian chast of 6 stories high and square in contour. Both arks carried all living things; man, woman, birds, beasts and creepy things. The sky filled with darkness and the heavenly storms wiped out all that roamed the earth. Despite both arks landing in the Middle East, Noah’s ark landed on Mt.Ararat, and Utnapishti’s ark on Mt.Nisir. For forty days and forty nights rain poured down on earth and on Noah’s ark, however, the Babylonian story was said to have rained for only seven days and seven nights. Utnapishtim and Noah individually sent two birds to find dry land with no avail. The third birds did not return, apparently finding dry land. Although both men released three birds, Utnapishtim released a dove, swallow and a raven; Noah released a raven once a... Free Essays on Flood Comparison Free Essays on Flood Comparison The Chaldean Flood Tablets, spawned from the city of Ur (Southern Iraq), depicts how the Babylonian God, Ea (a god who took part in the creation of man), rendered â€Å"the end of all flesh.† Nearly identical to the way God covered the earth with a great rain to spite the lawlessness of man. The biblical and the Babylonian interpretations resemble each other in many aspects though differ in distinct parts. Mankind became obnoxious, mendacious, and sinful in the eyes of God. Comparable to the Babylonian story, the people were looked upon as being too numerous and too noisy. God chose a righteous man named Noah to carry on the human race after The Great Flood. The Babylonian god Ea chose Utnapishtim to do the same, indirectly warning Utnapishtim of the great revelation through a dream. In contrast, Noah was forewarned directly through the instructions of God. Noah was told to build an ark of gopher wood and cover it inside and out with a pitch, just as Utnapishtim was told. The arks had an opening for daylight, a single door, along with numerous internal compartments. Noah’s ark was constructed 3 stories high and rectangular shaped opposed to the Babylonian chast of 6 stories high and square in contour. Both arks carried all living things; man, woman, birds, beasts and creepy things. The sky filled with darkness and the heavenly storms wiped out all that roamed the earth. Despite both arks landing in the Middle East, Noah’s ark landed on Mt.Ararat, and Utnapishti’s ark on Mt.Nisir. For forty days and forty nights rain poured down on earth and on Noah’s ark, however, the Babylonian story was said to have rained for only seven days and seven nights. Utnapishtim and Noah individually sent two birds to find dry land with no avail. The third birds did not return, apparently finding dry land. Although both men released three birds, Utnapishtim released a dove, swallow and a raven; Noah released a raven once a...

Lumbar puncture or Spinal anesthesia Essay Example | Topics and Well Written Essays - 1250 words

Lumbar puncture or Spinal anesthesia - Essay Example General anesthesia is a state of controlled unconsciousness during which patient doesn't feel anything and may be described as 'anesthetized'. During general anesthesia, anesthetic medications are injected into a vein, or anesthetic gases may be breathed into the lungs. When these medications are carried to the brain by the blood, they effectively "numb" the brain, and produce unconsciousness. Regional anesthesia is the specialized use of local anesthetic to numb a part of the body. Regional anesthesia can be used for operations on larger or deeper parts of the body. Local anesthetic drugs are injected near to the bundles of nerves which carry signals from that area of the body to the brain. The most common regional anesthetics (also known as regional 'blocks') are spinal and epidural anesthetics. These can be used for operations on the lower body. Use of anesthesia has been recorded since prehistory,where Opium poppy capsules were used in Sumeria and succeeding empires. Simple apparatus for smoking of opium were used for adminstration of anesthesia. Contries like India and China initially used cannabis incense and aconitum. As per the Book of Later Han, the physician Hua Tuo performed abdominal surgery using an anesthetic substance called mafeisan, which literally means cannabis boil powder, dissolved in wine. Other variants like Solanum species containing potent tropane alkaloids , Coca and Alcohol were also used in these initial days. The use of above herbal anaesthesia had a crucial drawback compared to modern practice in context of standardisation, as described on Wikepedia by Fallopus that "when soporifics are weak they are useless, and when strong, they kill". This drawback was tried to overcome by, standardizing production as much as possible, with taking production occurring from specific famous locations (such as opium from the fields of Thebes in ancient Egypt). Anaesthetics were sometimes administered in the spongia somnifera, a sponge into which a large quantity of drug was allowed to dry, from which a saturated solution could be trickled into the nose of the patient. At least in more recent centuries, trade was often highly standardized, with the drying and packing of opium in standard chests. Later, use of gases and vapours was also notice during mid 1800. Henry Hill Hickman experimented with carbon dioxide in the 1820s. British chemist Humphry Davy in 1799 used nitrous oxide .Effective general anessthesia for surgery begin in 1846, when a dentist, William Thomas Green Morton, gave diethyl ether at Massachusetts General Hospital, in the first public demonstration of diethyl ether as an anesthetic agent. It was then, Oliver Wendell Holmes, Sr. proposed naming the procedure ansthesia.Anesthesia gained good public repute when Queen Victoria accepted chloroform a la reine from Dr. John Snow during the birth of prince Leopold in 1857. Local anaesthesia originated with the

Problem Facing UK Copyright Law Arising in Digital Market Essay

Problem Facing UK Copyright Law Arising in Digital Market - Essay Example This paper will evaluate the Problems facing UK copyright law as a result of the digital market. Additionally, I will explain why I chose this topic. Moreover, the paper will cover my intended research methodology. Also, it will cover a review of the previous studies conducted on this topic. Many organizations and people come up with new innovations and inventions in addition to improving their current properties. This innovations, inventions and improvements have an economic value. As such, laws have been formulated to protect such intellectual properties and innovations against copying or illegal usage. These laws are founded on the concept or idea that if an individual, group or organisation has utilized his or her expertise, knowledge and resources to design and create a new item, no other person must benefit from that invention without the inventor’s authorization. In order to enforce compliance, there is need for legislation to deter illegal usage of another person’s property. Most governments enact copyright laws to guard and provide exclusive rights to creators of original work for a specified time. Often, it is referred to as the right to copy. However, it gives the copyright holder other additional rights. The development and creation of the computer network and digital media technologies have prompted the UK government to reinterpret the copy right laws. Patents, trade secrets and trademarks are applicable to expressible forms of information or ideas. Under the intellectual property law, owners are given exclusive rights to a multitude of intangible assets (Lemley, 2004). For example, literary, artistic works; inventions and discoveries, designs and symbols and musical works. Intellectual and copyright property rights encompass patents, trademarks, trade secrets and industrial design rights depending on the jurisdiction. Although most copyright laws in protecting property are territorial and confined to the area of their origin, a lot of